The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Austrian dose measurements onboard space station MIR and the International Space Station--overview and comparison.

The Atominstitute of the Austrian Universities has conducted various space research missions in the last 12 years in cooperation with the Institute for Biomedical Problems in Moscow. They dealt with the exact determination of the radiation hazards for cosmonauts and the development of precise measurement devices. Special emphasis will be laid on the last experiment on space station MIR the goal of which was the determination of the depth distribution of absorbed dose and dose equivalent in a water filled Phantom. The first results from dose measurements onboard the International Space Station (ISS) will also be discussed. The spherical Phantom with a diameter of 35 cm was developed at the Institute for Biomedical Problems and had 4 channels where dosimeters can be exposed in different depths. The exposure period covered the timeframe from May 1997 to February 1999. Thermoluminescent dosimeters (TLDs) were exposed inside the Phantom, either parallel or perpendicular to the hull of the spacecraft. For the evaluation of the linear energy transfer (LET), the high temperature ratio (HTR) method was applied. Based on this method a mean quality factor and, subsequently, the dose equivalent is calculated according to the Q(LET infinity) relationship proposed in ICRP 26. An increased contribution of neutrons could be detected inside the Phantom. However the total dose equivalent did not increase over the depth of the Phantom. As the first Austrian measurements on the ISS dosimeter packages were exposed for 248 days, starting in February 2001 at six different locations onboard the ISS. The Austrian dosimeter sets for this first exposure on the ISS contained five different kinds of passive thermoluminescent dosimeters. First results showed a position dependent absorbed dose rate at the ISS.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

A wavelet multiresolution analysis for spatio-temporal signals

The wavelet filters of the conventional 3D multiresolution analysis possess homogeneous spatial and temporal frequency characteristics which limits one's ability to match filter frequency characteristics to signal frequency behavior. Also, the conventional 3D multiresolution analysis employs an oct-tree decomposition structure which restricts the analysis of signal details to identical resolutions in space and time. This paper presents a 3D wavelet multiresolution analysis constructed from nonhomogeneous spatial and temporal filters, and an orthogonal sub-band coding scheme that decouples the spatial and temporal decomposition processes     

Simulation of fast ablation

A brief review of studies on simulation and control of a porous cooling process taking into account material transpiration is given. An approximated solution for an equation of the one-dimensional problem of fast ablation is also presented.     

Fiber optic fence sensor developments

Many detection technologies have been employed for perimeter detection, to sensitize a barrier. These outdoor perimeter fence detection sensors must reliably detect intruders attempting to cut or climb the barrier, while ignoring the effects of environmental noise, including nearby activity. In recent conference proceedings, the new IntelliFIBER/spl trade/ fiber optic based product was introduced and compared with previous technologies. IntelliFIBER is designed to provide the advantages of a nonconductive "dielectric" cable sensor, e.g., resistance to electromagnetic interference and the ability to provide longer cable zones. It utilizes the proven processor of the Intelli-FLEX/spl trade/ triboelectric cable fence sensor, which provides relay or bidirectional networked serial communications and is compatible with its calibration module. IntelliFIBER also capitalizes on the Intelli-FLEX's adaptive detection algorithms that were developed through extensive field-testing, to provide exceptional immunity to environmental alarms while still detecting the skilled intruder.     

Almost instant time inference for hybrid partially dynamic Bayesian networks

A Bayesian network (BN) is a compact representation for probabilistic models and inference. They have been used successfully for many military and civilian applications. It is well known that, in general, the inference algorithms to compute the exact a posterior probability of a target node given observed evidence are either computationally infeasible for dense networks or impossible for general hybrid networks. In those cases, one either computes the approximate results using stochastic simulation methods or approximates the model using discretization or a Gaussian mixture model before applying an exact inference algorithm. This paper combines the concept of simulation and model approximation to propose an efficient algorithm for those cases. The main contribution here is a unified treatment of arbitrary (nonlinear non-Gaussian) hybrid (discrete and continuous) BN inference having both computation and accuracy scalability. The key idea is to precompile the high-dimensional hybrid distribution using a hypercube representation and apply it for both static and dynamic BN inference. Since the inference process essentially becomes a combination of table look-up and some simple operations, the method is shown to be extremely efficient. It can also he scaled to achieve any desirable accuracy given sufficient preprocessing time and memory for the cases where exact inference is not possible     

Recent Breakthroughs in RF Photonics for Radar Systems

Radar systems require transmission of very high purity signals. Photonics is now mature enough to achieve analog transmission with very low noise, strong immunity, and wide-bandwidth even in harsh environments. We present our recent developments of optimized optical links dedicated to radar and multifunction systems     

Two Methods of Spectral Estimation for SARSAT Signals

Search and rescue satellite aided tracking (SARSAT) depends on the processing of emergency locator transmitter (ELT) signals which are received by a satellite in low polar orbit. Since the signal from a distressed vehicle is normally immersed in a background of other ELT signals (false alarms), interference, and noise, different methods of spectral estimation can provide advantages in estimating carrier frequency. A comparison between average spectrum and minimum spectrum for several real signals is provided here.     

Spiral evolution applied to legacy avionics systems

The US Department of Defense (DoD), through the Office of the Secretary of Defense (OSD), has determined that evolutionary acquisition is their strategy of choice for future software-intensive systems, and that the spiral development model (SDM) is the preferred method/process for software-intensive development life cycles. Electronic Systems Command (ESC) at Hanscom AFB, Massachusetts, has written a draft handbook on the use of Spiral Development for all future Command and Control (C2) systems, including reference to the DoD 5000 series and Air Force Program Directive 63-1, Acquisition System, which deploys this OSD mandate for future C2 systems. Barry Boehm's continued work on SDM which he conceived in the 1980s, is heavily biased toward development of new systems that are software-intensive, as noted in a workshop he gave at the Software Engineering Institute (SEI) in 2000.